Transplantation of Frozen Bovine Embryos

The biopolitics of frozen embryos.

The unresolved debate about frozen embryos has left open the discussion on "what to do with them". There are only three ways to deal with frozen embryos: 1) to leave them frozen indefinitely; 2) to defrost and discard them and 3) to use them for research. In this paper, we suggest that the application of current scientific knowledge, instead of inappropriately referring to ethical principles or...

P-42: Who Owns the Frozen Embryos?

Background: Success in assisted reproductive techniques is raising hope for many infertile couples, but as in these techniques many embryos are made, there is no choice other than cryopreservation of the not-transferred embryos. Along with development of these techniques, the number of In-vitro made embryos is rising and the number of transferred embryos is decreasing to prevent multiple gestat...

Developmental competence of bovine embryos reconstructed by the transfer of somatic cells derived from frozen tissues.

The ability of frozen-thawed fetal skin was examined to generate viable cell lines for nuclear transfer. Fetal skin frozen at -35 degrees C or -80 degrees C in the presence of 5% DMSO were used as tissue explants to generate somatic cells. The resultant confluent cells were then used as donors for nuclear transfer (NT). Of the bovine NT embryos reconstructed from the somatic cells, 78% to 81% s...

Viability of frozen-thawed bovine IVM/IVF embryos in relation to aging using various cryoprotectants.

Bovine IVF embryos developed on Days 7, 8 and 9 were equilibrated with 1.6 M propylene glycol (PG), 1.8 M ethylene glycol (EG), 1.1 M diethylene glycol (DEG) or 1.3 M ethylene glycol monomethyl ether (EME) for 10 to 20 min in modified phosphate buffered saline. (mPBS) supplemented with 10% superovulated cow serum. The embryos were loaded into 0.25-ml plastic straws and were placed directly into...

Frozen embryos: towards an equitable solution.